ABSTRACT
Objective:To establish the HPLC fingerprint of Centellae herba and determine the content of asiaticoside, madecassic acid and asiaticoside B simultaneously; To compare the quality differences of Centellae herba collected in different months. Methods:The chromatographic condition was a Shimadzu InertSustain C18 column (4.6 mm×250 mm, 5 μm) with a mobile phase consisting of acetonitrile and 2 mmol/L beta cyclodextrin in gradient elution at the flow rate of 0.8 ml/min. The detection wavelength was 204 nm, and the column temperature was 30 ℃. The different Centellae herba materials of collected in 2-12 months from Chenzhou were studied by the similarity evaluation combined with cluster analysis, principal component analysis and the three contents determination. Results:The HPLC fingerprint of Centellae herba was established and 9 common peaks were designated. The eleven samples were different, which can be aggregated into 4 categories and the quality of Centellae herba collected in July was the best. Conclusion:The established fingerprint and multi-components quantitative method are stable and reliable, which can provide a reference for the quality control and the utilization of Centellae herba resource.
ABSTRACT
Objective@#To study the effects of different compatibility ratios of Centella asiatica- Rhubarb on the dissolution rates of madecassoside and asiaticoside.@*Methods@#By using the HPLC method to analyze the content of madecassoside and asiaticoside in the Centella asiatica extract solution as well as Centella asiatica - Rhubarb extract solution with 6 different kinds of compatibility ratios. The tests were carried out by Thermo C18 (250 mm×4.6 mm, 5 μm) by isocratic elution with acetonitrile and 2 mmol/L of beta-cyclodextrin solution as mobile phase at a flow rate of 1 ml/min, the column temperature was 30 ℃ and the detection wavelength was 205 nm.@*Results@#Compared with the Centella asiatica extract solution, the dissolution rate of madecassoside decreased significantly with the increase of the proportion of rhubarb. The combination of Centella asiatica and Rhubarb had little effect on the content of asiaticoside.@*Conclusions@#Considering the clinical application and efficacy of Centella asiatica, the optimal ratio of Centella asiatica-Rhubarb is 10:1 and 15:1.
ABSTRACT
Objective: To separate the isomers of madecassoside in Centellae Herba and its total glucosides, determine madecassoside and asiaticoside in them, and establish an HPLC specific chromatogram for Centellae Herba total glucosides by using β-cyclodextrin as mobile phase additives. Methods: The Dikma Diamonsil™ C18 column (250 mm x 4. 6 mm, 5 μm) was served as analytical column and acetonitrile-2 mmol/L β-cyclodextrin (24 : 76) as mobile phase at a flow rate of 1. 0 mL/min, the detection wavelength was 205 nm and column temperature was 30°C. Theoretical plate number of asiaticoside was over 4 000. Results: The isomers in Centellae Herba and its total glucosides were separated with the additives of β-cyclodextrin, meeting the requirements of quantitative analysis. In which, madecassoside and asiaticoside showed a good linearity in the range of 0.181-18. 1 μg (r=0. 999 98) and 0.195-19. 5 μg (r=0. 999 98) each. The mean recovery of madecassoside and asiaticoside in Centellae Herba was 101. 2% and 101. 6% (n=9), and that of its 11 batches of total glucosides was 100. 3% and 99. 3%, respectively. Under the qualitative conditions, the six common peaks in HPLC specific chromatogram of its 11 batches of total glucosides could be used as marker peaks for qualitative identification. Conclusion: The analytical method is proved to be convenient and accurate, which can be used for the quality control of Centellae Herba and its total glucosides.